2gsp: Difference between revisions

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[[Image:2gsp.jpg|left|200px]]
{{Seed}}
[[Image:2gsp.png|left|200px]]


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{{STRUCTURE_2gsp|  PDB=2gsp  |  SCENE=  }}  
{{STRUCTURE_2gsp|  PDB=2gsp  |  SCENE=  }}  


'''RIBONUCLEASE T1/2',3'-CGPS AND 3'-GMP, 2 DAYS'''
===RIBONUCLEASE T1/2',3'-CGPS AND 3'-GMP, 2 DAYS===




==Overview==
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Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
The line below this paragraph, {{ABSTRACT_PUBMED_9546218}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9546218 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9546218}}


==About this Structure==
==About this Structure==
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[[Category: Endoribonuclease]]
[[Category: Endoribonuclease]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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