2oo4: Difference between revisions
New page: left|200px<br /> <applet load="2oo4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oo4, resolution 2.003Å" /> '''Structure of LNR-H... |
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[[Image:2oo4.gif|left|200px]]<br /> | [[Image:2oo4.gif|left|200px]]<br /><applet load="2oo4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="2oo4, resolution 2.003Å" /> | caption="2oo4, resolution 2.003Å" /> | ||
'''Structure of LNR-HD (Negative Regulatory Region) from human Notch 2'''<br /> | '''Structure of LNR-HD (Negative Regulatory Region) from human Notch 2'''<br /> | ||
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==Overview== | ==Overview== | ||
Notch receptors transmit signals between adjacent cells. Signaling is, initiated when ligand binding induces metalloprotease cleavage of Notch, within an extracellular negative regulatory region (NRR). We present here, the X-ray structure of the human NOTCH2 NRR, which adopts an autoinhibited, conformation. Extensive interdomain interactions within the NRR bury the, metalloprotease site, showing that a substantial conformational movement, is necessary to expose this site during activation by ligand., Leukemia-associated mutations in NOTCH1 probably release autoinhibition by, destabilizing the conserved hydrophobic core of the NRR. | Notch receptors transmit signals between adjacent cells. Signaling is, initiated when ligand binding induces metalloprotease cleavage of Notch, within an extracellular negative regulatory region (NRR). We present here, the X-ray structure of the human NOTCH2 NRR, which adopts an autoinhibited, conformation. Extensive interdomain interactions within the NRR bury the, metalloprotease site, showing that a substantial conformational movement, is necessary to expose this site during activation by ligand., Leukemia-associated mutations in NOTCH1 probably release autoinhibition by, destabilizing the conserved hydrophobic core of the NRR. | ||
==About this Structure== | ==About this Structure== | ||
2OO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA, ZN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2OO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OO4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: alpha-beta-sandwich; sea domain; lnr; lin12 notch repeat; cysteine-rich; hd domain]] | [[Category: alpha-beta-sandwich; sea domain; lnr; lin12 notch repeat; cysteine-rich; hd domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:59:03 2008'' | ||
Revision as of 12:59, 23 January 2008
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Structure of LNR-HD (Negative Regulatory Region) from human Notch 2
Overview
Notch receptors transmit signals between adjacent cells. Signaling is, initiated when ligand binding induces metalloprotease cleavage of Notch, within an extracellular negative regulatory region (NRR). We present here, the X-ray structure of the human NOTCH2 NRR, which adopts an autoinhibited, conformation. Extensive interdomain interactions within the NRR bury the, metalloprotease site, showing that a substantial conformational movement, is necessary to expose this site during activation by ligand., Leukemia-associated mutations in NOTCH1 probably release autoinhibition by, destabilizing the conserved hydrophobic core of the NRR.
About this Structure
2OO4 is a Single protein structure of sequence from Homo sapiens with CA, ZN and GOL as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for autoinhibition of Notch., Gordon WR, Vardar-Ulu D, Histen G, Sanchez-Irizarry C, Aster JC, Blacklow SC, Nat Struct Mol Biol. 2007 Apr;14(4):295-300. Epub 2007 Apr 1. PMID:17401372
Page seeded by OCA on Wed Jan 23 14:59:03 2008
