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| [[Image:2h1k.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2h1k| PDB=2h1k | SCENE= }} | | {{STRUCTURE_2h1k| PDB=2h1k | SCENE= }} |
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| '''Crystal structure of the Pdx1 homeodomain in complex with DNA'''
| | ===Crystal structure of the Pdx1 homeodomain in complex with DNA=== |
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| ==Overview==
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| Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of non-insulin-dependent diabetes mellitus, MODY-4. We demonstrate that the homeodomain reproduces the binding specificity of the full-length protein. We report the 2.4 A resolution crystal structure of the homeodomain bound to a target DNA. The two Pdx1/DNA complexes in the asymmetric unit display conformational differences: in the DNA curvature, the orientation of the homeodomain in the major groove, and the order of the N-terminal arm. Comparing the two complexes indicates invariant protein-DNA contacts, and variant contacts that are unique to each binding orientation. An induced fit model is proposed that depends on the DNA conformation and provides a mechanism for nonlocal contributions to binding specificity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17315980}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17315980 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17315980}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rose, R B.]] | | [[Category: Rose, R B.]] |
| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:45:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:20:32 2008'' |
