2h39: Difference between revisions

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[[Image:2h39.gif|left|200px]]
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[[Image:2h39.png|left|200px]]


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{{STRUCTURE_2h39|  PDB=2h39  |  SCENE=  }}  
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'''Crystal Structure of an ADP-Glucose Phosphorylase from Arabidopsis thaliana with bound ADP-Glucose'''
===Crystal Structure of an ADP-Glucose Phosphorylase from Arabidopsis thaliana with bound ADP-Glucose===


==Overview==
The X-ray crystal structure of the At5g18200.1 protein has been determined to a nominal resolution of 2.30 A. The structure has a histidine triad (HIT)-like fold containing two distinct HIT-like motifs. The sequence of At5g18200.1 indicates a distant family relationship to the Escherichia coli galactose-1-P uridylyltransferase (GalT): the determined structure of the At5g18200.1 protein confirms this relationship. The At5g18200.1 protein does not demonstrate GalT activity but instead catalyzes adenylyl transfer in the reaction of ADP-glucose with various phosphates. The best acceptor among those evaluated is phosphate itself; thus, the At5g18200.1 enzyme appears to be an ADP-glucose phosphorylase. The enzyme catalyzes the exchange of (14)C between ADP-[(14)C]glucose and glucose-1-P in the absence of phosphate. The steady state kinetics of exchange follows the ping-pong bi-bi kinetic mechanism, with a k(cat) of 4.1 s(-)(1) and K(m) values of 1.4 and 83 microM for ADP-[(14)C]glucose and glucose-1-P, respectively, at pH 8.5 and 25 degrees C. The overall reaction of ADP-glucose with phosphate to produce ADP and glucose-1-P follows ping-pong bi-bi steady state kinetics, with a k(cat) of 2.7 s(-)(1) and K(m) values of 6.9 and 90 microM for ADP-glucose and phosphate, respectively, at pH 8.5 and 25 degrees C. The kinetics are consistent with a double-displacement mechanism that involves a covalent adenylyl-enzyme intermediate. The X-ray crystal structure of this intermediate was determined to 1.83 A resolution and shows the AMP group bonded to His(186). The value of K(eq) in the direction of ADP and glucose-1-P formation is 5.0 at pH 7.0 and 25 degrees C in the absence of a divalent metal ion, and it is 40 in the presence of 1 mM MgCl(2).


==About this Structure==
==About this Structure==
2H39 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gdk 2gdk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H39 OCA].  
2H39 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gdk 2gdk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H39 OCA].  


==Reference==
==Reference==
Structure and mechanism of an ADP-glucose phosphorylase from Arabidopsis thaliana., McCoy JG, Arabshahi A, Bitto E, Bingman CA, Ruzicka FJ, Frey PA, Phillips GN Jr, Biochemistry. 2006 Mar 14;45(10):3154-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16519510 16519510]
<ref group="xtra">PMID:16519510</ref><references group="xtra"/>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]
[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]
[[Category: Bingman, C A.]]
[[Category: Bingman, C A.]]
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[[Category: Psi]]
[[Category: Psi]]
[[Category: Structural genomics functional follow-up study]]
[[Category: Structural genomics functional follow-up study]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:43:15 2009''

Revision as of 18:43, 17 February 2009

File:2h39.png

Template:STRUCTURE 2h39

Crystal Structure of an ADP-Glucose Phosphorylase from Arabidopsis thaliana with bound ADP-Glucose

About this Structure

2H39 is a 2 chains structure of sequences from Arabidopsis thaliana. This structure supersedes the now removed PDB entry 2gdk. Full crystallographic information is available from OCA.

Reference

  1. McCoy JG, Arabshahi A, Bitto E, Bingman CA, Ruzicka FJ, Frey PA, Phillips GN Jr. Structure and mechanism of an ADP-glucose phosphorylase from Arabidopsis thaliana. Biochemistry. 2006 Mar 14;45(10):3154-62. PMID:16519510 doi:10.1021/bi052232m

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