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| {{STRUCTURE_2h7o| PDB=2h7o | SCENE= }} | | {{STRUCTURE_2h7o| PDB=2h7o | SCENE= }} |
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| '''Crystal structure of the Rho-GTPase binding domain of YpkA'''
| | ===Crystal structure of the Rho-GTPase binding domain of YpkA=== |
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| ==Overview==
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| Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16959567}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16959567 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16959567}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Yopo]] | | [[Category: Yopo]] |
| [[Category: Ypka]] | | [[Category: Ypka]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:58:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:29:59 2008'' |
