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| {{STRUCTURE_2hcs| PDB=2hcs | SCENE= }} | | {{STRUCTURE_2hcs| PDB=2hcs | SCENE= }} |
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| '''Crystal structure of RNA dependant RNA polymerase domain of West Nile virus'''
| | ===Crystal structure of RNA dependant RNA polymerase domain of West Nile virus=== |
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| ==Overview==
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| Viruses of the family Flaviviridae are important human and animal pathogens. Among them, the Flaviviruses dengue (DENV) and West Nile (WNV) cause regular outbreaks with fatal outcomes. The RNA-dependent RNA polymerase (RdRp) activity of the non-structural protein 5 (NS5) is a key activity for viral RNA replication. In this study, crystal structures of enzymatically active and inactive WNV RdRp domains were determined at 3.0- and 2.35-A resolution, respectively. The determined structures were shown to be mostly similar to the RdRps of the Flaviviridae members hepatitis C and bovine viral diarrhea virus, although with unique elements characteristic for the WNV RdRp. Using a reverse genetic system, residues involved in putative interactions between the RNA-cap methyltransferase (MTase) and the RdRp domain of Flavivirus NS5 were identified. This allowed us to propose a model for the structure of the full-length WNV NS5 by in silico docking of the WNV MTase domain (modeled from our previously determined structure of the DENV MTase domain) onto the RdRp domain. The Flavivirus RdRp domain structure determined here should facilitate both the design of anti-Flavivirus drugs and structure-function studies of the Flavivirus replication complex in which the multifunctional NS5 protein plays a central role.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17287213}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17287213 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17287213}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Vizier]] | | [[Category: Vizier]] |
| [[Category: West-nile virus rna polymerase]] | | [[Category: West-nile virus rna polymerase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:07:54 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:44:28 2008'' |
