2hdx: Difference between revisions

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[[Image:2hdx.gif|left|200px]]
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{{STRUCTURE_2hdx|  PDB=2hdx  |  SCENE=  }}  
{{STRUCTURE_2hdx|  PDB=2hdx  |  SCENE=  }}  


'''Crystal structure of the Src homology-2 domain of SH2-B in complex with Jak2 pTyr813 phosphopeptide'''
===Crystal structure of the Src homology-2 domain of SH2-B in complex with Jak2 pTyr813 phosphopeptide===




==Overview==
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SH2-B, APS, and Lnk constitute a family of adapter proteins that modulate signaling by protein tyrosine kinases. These adapters contain an N-terminal dimerization region, a pleckstrin homology domain, and a C-terminal Src homology-2 (SH2) domain. SH2-B is recruited via its SH2 domain to various protein tyrosine kinases, including Janus kinase-2 (Jak2) and the insulin receptor. Here, we present the crystal structure at 2.35 A resolution of the SH2 domain of SH2-B in complex with a phosphopeptide representing the SH2-B recruitment site in Jak2 (pTyr813). The structure reveals a canonical SH2 domain-phosphopeptide binding mode, but with specific recognition of a glutamate at the +1 position relative to phosphotyrosine, in addition to recognition of a hydrophobic residue at the +3 position. Biochemical studies of SH2-B and APS demonstrate that, although the SH2 domains of these two adapter proteins share 79% sequence identity, the SH2-B SH2 domain binds preferentially to Jak2, whereas the APS SH2 domain has higher affinity for the insulin receptor. This differential specificity is attributable to the difference in the oligomeric states of the two SH2 domains: monomeric for SH2-B and dimeric for APS.
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{{ABSTRACT_PUBMED_16824542}}


==About this Structure==
==About this Structure==
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[[Category: Phosphotyrosine]]
[[Category: Phosphotyrosine]]
[[Category: Sh2]]
[[Category: Sh2]]
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Revision as of 14:53, 28 July 2008

File:2hdx.png

Template:STRUCTURE 2hdx

Crystal structure of the Src homology-2 domain of SH2-B in complex with Jak2 pTyr813 phosphopeptide

Template:ABSTRACT PUBMED 16824542

About this Structure

2HDX is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for phosphotyrosine recognition by the Src homology-2 domains of the adapter proteins SH2-B and APS., Hu J, Hubbard SR, J Mol Biol. 2006 Aug 4;361(1):69-79. Epub 2006 Jun 16. PMID:16824542

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