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| {{STRUCTURE_2het| PDB=2het | SCENE= }} | | {{STRUCTURE_2het| PDB=2het | SCENE= }} |
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| '''Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3'''
| | ===Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3=== |
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| ==Overview==
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| Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17015448}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17015448 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17015448}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Helix-loop-helix]] | | [[Category: Helix-loop-helix]] |
| [[Category: Recoverin]] | | [[Category: Recoverin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:12:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:41:51 2008'' |
