2hhc: Difference between revisions

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[[Image:2hhc.jpg|left|200px]]
{{Seed}}
[[Image:2hhc.png|left|200px]]


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{{STRUCTURE_2hhc|  PDB=2hhc  |  SCENE=  }}  
{{STRUCTURE_2hhc|  PDB=2hhc  |  SCENE=  }}  


'''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''
===Crystal structure of fucosyltransferase NodZ from Bradyrhizobium===




==Overview==
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The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17762900 17762900]
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17762900 17762900]
Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747720 14747720]
Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase., Quesada-Vincens D, Fellay R, Nasim T, Viprey V, Burger U, Prome JC, Broughton WJ, Jabbouri S, J Bacteriol. 1997 Aug;179(16):5087-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9260950 9260950]
Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin., Quinto C, Wijfjes AH, Bloemberg GV, Blok-Tip L, Lopez-Lara IM, Lugtenberg BJ, Thomas-Oates JE, Spaink HP, Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4336-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9113990 9113990]
[[Category: Bradyrhizobium sp.]]
[[Category: Bradyrhizobium sp.]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Nodulation]]
[[Category: Nodulation]]
[[Category: Nodz]]
[[Category: Nodz]]
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