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| {{STRUCTURE_2hi2| PDB=2hi2 | SCENE= }} | | {{STRUCTURE_2hi2| PDB=2hi2 | SCENE= }} |
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| '''Crystal structure of native Neisseria gonorrhoeae Type IV pilin at 2.3 Angstroms Resolution'''
| | ===Crystal structure of native Neisseria gonorrhoeae Type IV pilin at 2.3 Angstroms Resolution=== |
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| ==Overview==
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| Type IV pili (T4P) are long, thin, flexible filaments on bacteria that undergo assembly-disassembly from inner membrane pilin subunits and exhibit astonishing multifunctionality. Neisseria gonorrhoeae (gonococcal or GC) T4P are prototypic virulence factors and immune targets for increasingly antibiotic-resistant human pathogens, yet detailed structures are unavailable for any T4P. Here, we determined a detailed experimental GC-T4P structure by quantitative fitting of a 2.3 A full-length pilin crystal structure into a 12.5 A resolution native GC-T4P reconstruction solved by cryo-electron microscopy (cryo-EM) and iterative helical real space reconstruction. Spiraling three-helix bundles form the filament core, anchor the globular heads, and provide strength and flexibility. Protruding hypervariable loops and posttranslational modifications in the globular head shield conserved functional residues in pronounced grooves, creating a surprisingly corrugated pilus surface. These results clarify T4P multifunctionality and assembly-disassembly while suggesting unified assembly mechanisms for T4P, archaeal flagella, and type II secretion system filaments.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16949362}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16949362 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16949362}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Membrane protein]] | | [[Category: Membrane protein]] |
| [[Category: Type iv pilin]] | | [[Category: Type iv pilin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:19:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:32:48 2008'' |
