2hi9: Difference between revisions

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[[Image:2hi9.jpg|left|200px]]
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[[Image:2hi9.png|left|200px]]


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{{STRUCTURE_2hi9|  PDB=2hi9  |  SCENE=  }}  
{{STRUCTURE_2hi9|  PDB=2hi9  |  SCENE=  }}  


'''Crystal Structure of human native protein C inhibitor'''
===Crystal Structure of human native protein C inhibitor===




==Overview==
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Protein C inhibitor (PCI) is a multifunctional serpin with wide ranging protease inhibitory functions, unique cofactor binding activities, and potential non-inhibitory functions akin to the hormone-transporting serpins. To gain insight into the molecular mechanisms utilized by PCI we developed a robust expression system in Escherichia coli and solved the crystal structure of PCI in its native state. The five monomers obtained from our two crystal forms provide an NMR-like ensemble revealing regions of inherent flexibility. The reactive center loop (RCL) of PCI is long and highly flexible with no evidence of hinge region incorporation into beta-sheet A, as seen for other heparin-binding serpins. We adapted an extrinsic fluorescence method for determining dissociation constants for heparin and find that the N-terminal tail of PCI and residues adjacent to helix H are not involved in heparin binding. The minimal heparin length capable of tight binding to PCI was determined to be chains of eight monosaccharide units. A large hydrophobic pocket occupied by hydrophobic crystal contacts was found in an analogous position to the hormone-binding site in thyroxine-binding globulin. In conclusion, the data presented here provide important insights into the mechanisms by which PCI exercises its multiple inhibitory and non-inhibitory functions.
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==Disease==
==Disease==
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[[Category: Serpin]]
[[Category: Serpin]]
[[Category: Thrombin inhibitor]]
[[Category: Thrombin inhibitor]]
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Revision as of 05:33, 29 July 2008

File:2hi9.png

Template:STRUCTURE 2hi9

Crystal Structure of human native protein C inhibitor

Template:ABSTRACT PUBMED 17337440

Disease

Known disease associated with this structure: Protein C inhibitor deficiency OMIM:[601841]

About this Structure

2HI9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of native protein C inhibitor provides insight into its multiple functions., Li W, Adams TE, Kjellberg M, Stenflo J, Huntington JA, J Biol Chem. 2007 May 4;282(18):13759-68. Epub 2007 Mar 2. PMID:17337440

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