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| [[Image:2hii.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2hii| PDB=2hii | SCENE= }} | | {{STRUCTURE_2hii| PDB=2hii | SCENE= }} |
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| '''heterotrimeric PCNA sliding clamp'''
| | ===heterotrimeric PCNA sliding clamp=== |
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| ==Overview==
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| DNA sliding clamps encircle DNA and provide binding sites for many DNA-processing enzymes. However, it is largely unknown how sliding clamps like proliferating cell nuclear antigen (PCNA) coordinate multistep DNA transactions. We have determined structures of Sulfolobus solfataricus DNA ligase and heterotrimeric PCNA separately by X-ray diffraction and in complex by small-angle X-ray scattering (SAXS). Three distinct PCNA subunits assemble into a protein ring resembling the homotrimeric PCNA of humans but with three unique protein-binding sites. In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has an open, extended conformation. When complexed with heterotrimeric PCNA, the DNA ligase binds to the PCNA3 subunit and ligase retains an open, extended conformation. A closed, ring-shaped conformation of ligase catalyzes a DNA end-joining reaction that is strongly stimulated by PCNA. This open-to-closed switch in the conformation of DNA ligase is accommodated by a malleable interface with PCNA that serves as an efficient platform for DNA ligation.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17052461}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17052461 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17052461}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Processivity factor]] | | [[Category: Processivity factor]] |
| [[Category: Sliding clamp]] | | [[Category: Sliding clamp]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:20:11 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:00:02 2008'' |
