2hou: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2hou.gif|left|200px]]
{{Seed}}
[[Image:2hou.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hou|  PDB=2hou  |  SCENE=  }}  
{{STRUCTURE_2hou|  PDB=2hou  |  SCENE=  }}  


'''Structure ensembles of duplex DNA containing a 4'-oxidized abasic site.'''
===Structure ensembles of duplex DNA containing a 4'-oxidized abasic site.===




==Overview==
<!--  
A 4'-oxidized abasic site (X) has been synthesized in a defined duplex DNA sequence, 5'-d(CCAAAGXACCGGG)-3'/3'-d(GGTTTCATGGCCC)-5' (1). Its structure has been determined by two-dimensional NMR methods, molecular modeling, and molecular dynamics simulations. 1 is globally B-form with the base (A) opposite X intrahelical and well-stacked. Only the alpha anomer of X is observed, and the abasic site deoxyribose is largely intrahelical. These results are compared with a normal abasic site (Y) in the same sequence context (2). Y is composed of a 60:40 mixture of alpha and beta anomers (2alpha and 2beta). In both 2alpha and 2beta, the base (A) opposite Y is intrahelical and well-stacked and the abasic site deoxyribose is predominantly extrahelical, consistent with the reported structures of the normal abasic site in a similar sequence context [Hoehn, S. T., Turner, C. J., and Stubbe, J. (2001) Nucleic Acids Res. 29, 3413-3423]. Molecular dynamics simulations reveal that the normal abasic site appears to be conformationally more flexible than the 4'-oxidized abasic site. The importance of the structure and flexibility of the abasic site in the recognition by the DNA repair enzyme Ape1 is discussed.
The line below this paragraph, {{ABSTRACT_PUBMED_17323932}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17323932 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_17323932}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOU OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOU OCA].  


==Reference==
==Reference==
Line 29: Line 33:
[[Category: Bleomycin]]
[[Category: Bleomycin]]
[[Category: Dna lesion]]
[[Category: Dna lesion]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:31:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:29:14 2008''

Revision as of 16:29, 27 July 2008

File:2hou.png

Template:STRUCTURE 2hou

Structure ensembles of duplex DNA containing a 4'-oxidized abasic site.

Template:ABSTRACT PUBMED 17323932

About this Structure

Full experimental information is available from OCA.

Reference

Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites., Chen J, Dupradeau FY, Case DA, Turner CJ, Stubbe J, Biochemistry. 2007 Mar 20;46(11):3096-107. Epub 2007 Feb 27. PMID:17323932

Page seeded by OCA on Sun Jul 27 19:29:14 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2hou&oldid=663154"