2hp4: Difference between revisions

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[[Image:2hp4.~~jpg~~|left|200px]]

[[Image:2hp4.png|left|200px]]

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~~'''~~Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor~~'''~~

===Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor===

~~==Overview==~~

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~~Human CD8 is a T cell coreceptor~~, ~~which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation~~, ~~both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 alphaalpha are required for efficient inhibition. Based upon our knowledge of~~ the ~~wild-type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 alphaalpha that binds~~ to ~~pHLA I with approximately fourfold higher affinity. We have characterized~~ the ~~binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure~~ at 2.~~1 A resolution using X-ray crystallography~~. ~~The analysis of this structure suggests that the higher affinity~~ is ~~achieved by providing a larger side chain that allows for an optimal contact to be made between~~ the ~~HLA alpha3 loop and the mutated CDR~~-~~like loops of CD8.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17243170}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17243170 is the PubMed ID number.

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==About this Structure==

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[[Category: Protein engineering]]

[[Category: Soluble protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 06~~:32:15 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:51:04 2008''

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-[[Image:2hp4.jpg|left|200px]]
+{{Seed}}
+[[Image:2hp4.png|left|200px]]
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 {{STRUCTURE_2hp4|  PDB=2hp4  |  SCENE=  }}
-'''Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor'''
+===Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor===
-==Overview==
+<!--
-Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 alphaalpha are required for efficient inhibition. Based upon our knowledge of the wild-type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 alphaalpha that binds to pHLA I with approximately fourfold higher affinity. We have characterized the binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure at 2.1 A resolution using X-ray crystallography. The analysis of this structure suggests that the higher affinity is achieved by providing a larger side chain that allows for an optimal contact to be made between the HLA alpha3 loop and the mutated CDR-like loops of CD8.
+The line below this paragraph, {{ABSTRACT_PUBMED_17243170}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17243170 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17243170}}
 ==About this Structure==
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 [[Category: Protein engineering]]
 [[Category: Soluble protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:32:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:51:04 2008''