2ihe: Difference between revisions

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[[Image:2ihe.~~jpg~~|left|200px]]

[[Image:2ihe.png|left|200px]]

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~~'''~~Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus~~'''~~

===Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus===

~~==Overview==~~

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~~In contrast to the majority of tetrameric SSB proteins~~, the ~~recently discovered SSB proteins from~~ the ~~Thermus~~/~~Deinoccus group form dimers~~. ~~We solved the crystal structures of the SSB protein from Thermus aquaticus (TaqSSB~~) ~~and a deletion mutant of~~ the ~~protein and show the structure of their ssDNA binding domains to be similar to the structure of tetrameric SSBs~~. ~~Two conformations accompanied by proline cis~~-~~trans isomerization are observed in the flexible C~~-terminal region. For the first time, we were able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein. This highly conserved region is essential for interaction with other proteins and we show it to adopt an extended conformation devoid of secondary structure. A model for binding this region to the chi subunit of DNA polymerase III is proposed. It explains at a molecular level the reason for the ssb113 phenotype observed in Escherichia coli.

The line below this paragraph, {{ABSTRACT_PUBMED_17148487}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17148487 is the PubMed ID number.

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==About this Structure==

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[[Category: Protein-protein interaction]]

[[Category: Thermophile organism]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 07~~:30:21 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:22:50 2008''

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-[[Image:2ihe.jpg|left|200px]]
+{{Seed}}
+[[Image:2ihe.png|left|200px]]
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 {{STRUCTURE_2ihe|  PDB=2ihe  |  SCENE=  }}
-'''Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus'''
+===Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus===
-==Overview==
+<!--
-In contrast to the majority of tetrameric SSB proteins, the recently discovered SSB proteins from the Thermus/Deinoccus group form dimers. We solved the crystal structures of the SSB protein from Thermus aquaticus (TaqSSB) and a deletion mutant of the protein and show the structure of their ssDNA binding domains to be similar to the structure of tetrameric SSBs. Two conformations accompanied by proline cis-trans isomerization are observed in the flexible C-terminal region. For the first time, we were able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein. This highly conserved region is essential for interaction with other proteins and we show it to adopt an extended conformation devoid of secondary structure. A model for binding this region to the chi subunit of DNA polymerase III is proposed. It explains at a molecular level the reason for the ssb113 phenotype observed in Escherichia coli.
+The line below this paragraph, {{ABSTRACT_PUBMED_17148487}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17148487 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17148487}}
 ==About this Structure==
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 [[Category: Protein-protein interaction]]
 [[Category: Thermophile organism]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:30:21 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:22:50 2008''