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| {{STRUCTURE_2ihs| PDB=2ihs | SCENE= }} | | {{STRUCTURE_2ihs| PDB=2ihs | SCENE= }} |
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| '''Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide'''
| | ===Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide=== |
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| ==Overview==
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| B30.2/SPRY domains are found in numerous proteins that cover a wide spectrum of biological functions, including regulation of cytokine signaling and innate retroviral restriction. Herein, we report the crystal structure of the B30.2/SPRY domain of a SPRY domain-containing SOCS box (SSB) protein, GUSTAVUS, complexed with a 20 amino acid peptide derived from the RNA helicase VASA, revealing how these domains recognize target proteins. The peptide-binding site is conformationally rigid and has a preformed pocket. The interaction between the pocket and the Asp-Ile-Asn-Asn-Asn-Asn sequence within the peptide accounts for the high-affinity binding between GUSTAVUS and VASA. This observation led to a facile identification of the Glu-Leu-Asn-Asn-Asn-Leu sequence as the recognition motif in a proapoptotic protein Par-4 for its interaction with a GUSTAVUS homolog, SSB-1. Ensuing analyses indicated that many B30.2/SPRY domains have a similar preformed pocket, which would allow them to bind multiple targets.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17189197}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17189197 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17189197}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Trim family]] | | [[Category: Trim family]] |
| [[Category: Vasa]] | | [[Category: Vasa]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:31:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:26:37 2008'' |
