2ip6: Difference between revisions

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[[Image:2ip6.jpg|left|200px]]
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[[Image:2ip6.png|left|200px]]


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{{STRUCTURE_2ip6|  PDB=2ip6  |  SCENE=  }}  
{{STRUCTURE_2ip6|  PDB=2ip6  |  SCENE=  }}  


'''Crystal structure of PedB'''
===Crystal structure of PedB===




==Overview==
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BACKGROUND: Pediocin-like bacteriocins, ribosomally-synthesized antimicrobial peptides, are generally coexpressed with cognate immunity proteins in order to protect the bacteriocin-producer from its own bacteriocin. As a step for understanding the mode of action of immunity proteins, we determined the crystal structure of PedB, a pediocin-like immunity protein conferring immunity to pediocin PP-1. RESULTS: The 1.6 A crystal structure of PedB reveals that PedB consists of an antiparallel four-helix bundle with a flexible C-terminal end. PedB shows structural similarity to an immunity protein against enterocin A (EntA-im) but some disparity to an immunity protein against carnobacteriocin B2 (ImB2) in both the C-terminal conformation and the local structure constructed by alpha3, alpha4, and their connecting loop. Structure-inspired mutational studies reveal that deletion of the last seven residues of the C-terminus of PedB almost abolished its immunity activity. CONCLUSION: The fact that PedB, EntA-im, and ImB2 share a four-helix bundle structure strongly suggests the structural conservation of this motif in the pediocin-like immunity proteins. The significant difference in the core structure and the C-terminal conformation provides a structural basis for the classification of pediocin-like immunity proteins. Our mutational study using C-terminal-shortened PedBs and the investigation of primary sequence of the C-terminal region, propose that several polar or charged residues in the extreme C-terminus of PedB which is crucial for the immunity are involved in the specific recognition of pediocin PP-1.
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{{ABSTRACT_PUBMED_17537233}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
High resolution crystal structure of PedB: a structural basis for the classification of pediocin-like immunity proteins., Kim IK, Kim MK, Kim JH, Yim HS, Cha SS, Kang SO, BMC Struct Biol. 2007 May 30;7:35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17537233 17537233]
High resolution crystal structure of PedB: a structural basis for the classification of pediocin-like immunity proteins., Kim IK, Kim MK, Kim JH, Yim HS, Cha SS, Kang SO, BMC Struct Biol. 2007 May 30;7:35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17537233 17537233]
Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 A resolution., Kim IK, Kim MK, Yim HS, Cha SS, Kang SO, Biochim Biophys Acta. 2005 Aug 10;1751(2):205-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16019271 16019271]
[[Category: Pediococcus pentosaceus]]
[[Category: Pediococcus pentosaceus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Antimicrobial protein]]
[[Category: Antimicrobial protein]]
[[Category: Immunity protein to the pediocin pp-1]]
[[Category: Immunity protein to the pediocin pp-1]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:44:17 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:46:09 2008''

Revision as of 15:46, 27 July 2008

File:2ip6.png

Template:STRUCTURE 2ip6

Crystal structure of PedB

Template:ABSTRACT PUBMED 17537233

About this Structure

2IP6 is a Single protein structure of sequence from Pediococcus pentosaceus. Full crystallographic information is available from OCA.

Reference

High resolution crystal structure of PedB: a structural basis for the classification of pediocin-like immunity proteins., Kim IK, Kim MK, Kim JH, Yim HS, Cha SS, Kang SO, BMC Struct Biol. 2007 May 30;7:35. PMID:17537233

Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 A resolution., Kim IK, Kim MK, Yim HS, Cha SS, Kang SO, Biochim Biophys Acta. 2005 Aug 10;1751(2):205-8. PMID:16019271

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