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| {{STRUCTURE_2iu5| PDB=2iu5 | SCENE= }} | | {{STRUCTURE_2iu5| PDB=2iu5 | SCENE= }} |
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| '''DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS'''
| | ===DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS=== |
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| ==Overview==
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| Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16760471}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16760471 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16760471}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Synthase]] | | [[Category: Synthase]] |
| [[Category: Tetr family]] | | [[Category: Tetr family]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:53:01 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:30:43 2008'' |
