2j19: Difference between revisions

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[[Image:2j19.jpg|left|200px]]
{{Seed}}
[[Image:2j19.png|left|200px]]


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{{STRUCTURE_2j19|  PDB=2j19  |  SCENE=  }}  
{{STRUCTURE_2j19|  PDB=2j19  |  SCENE=  }}  


'''FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)'''
===FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)===




==Overview==
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The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.
The line below this paragraph, {{ABSTRACT_PUBMED_17211068}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_17211068}}


==About this Structure==
==About this Structure==
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[[Category: Peroxidase]]
[[Category: Peroxidase]]
[[Category: Pyrrolidone carboxylic acid]]
[[Category: Pyrrolidone carboxylic acid]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:12:30 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:15:48 2008''

Revision as of 19:15, 28 July 2008

File:2j19.png

Template:STRUCTURE 2j19

FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)

Template:ABSTRACT PUBMED 17211068

About this Structure

2J19 is a Single protein structure of sequence from Leptoxyphium fumago. Full crystallographic information is available from OCA.

Reference

Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068

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