3hck: Difference between revisions
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New page: left|200px<br /> <applet load="3hck" size="450" color="white" frame="true" align="right" spinBox="true" caption="3hck" /> '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK S... |
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[[Image:3hck.gif|left|200px]]<br /> | [[Image:3hck.gif|left|200px]]<br /><applet load="3hck" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="3hck" size=" | |||
caption="3hck" /> | caption="3hck" /> | ||
'''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES'''<br /> | '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
The hematopoietic cellular kinase (Hck) is a member of the Src family of | The hematopoietic cellular kinase (Hck) is a member of the Src family of non-receptor protein-tyrosine kinases and participates in signal transduction events regulating the growth, differentiation and function of phagocytes. The secondary structure of the SH2 domain for Hck was determined for a 13C/15N-enriched sample using multi-dimensional NMR spectroscopy. The secondary structure for the domain was determined from chemical shift indices [1H alpha, 13C alpha and 13C'], sequential NOEs [d(alphaN)(i, i+1) and d(NN)(i, i+1)], and 3J(alphaN) scalar coupling constants. The Hck SH2 domain consists of two alpha-helices and seven beta-strands. Complementary strands of beta-sheets were identified from long-range NOEs using a novel 3D, 13C/15N-edited HMQC-NOESY-(HCACO)NH experiment that correlated 1H alpha resonances between beta-strands. The secondary structure for Hck SH2 is similar to that predicted from the sequence alignment of the Src-family protein tyrosine kinases. | ||
==About this Structure== | ==About this Structure== | ||
3HCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 3HCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gmeiner, W | [[Category: Gmeiner, W H.]] | ||
[[Category: Smithgall, T | [[Category: Smithgall, T E.]] | ||
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: hck]] | [[Category: hck]] | ||
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[[Category: tyrosine kinase]] | [[Category: tyrosine kinase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:09:42 2008'' | ||
