2jc2: Difference between revisions

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[[Image:2jc2.jpg|left|200px]]
{{Seed}}
[[Image:2jc2.png|left|200px]]


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{{STRUCTURE_2jc2|  PDB=2jc2  |  SCENE=  }}  
{{STRUCTURE_2jc2|  PDB=2jc2  |  SCENE=  }}  


'''THE CRYSTAL STRUCTURE OF THE NATURAL F112L HUMAN SORCIN MUTANT'''
===THE CRYSTAL STRUCTURE OF THE NATURAL F112L HUMAN SORCIN MUTANT===




==Overview==
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The penta-EF hand protein sorcin participates in the modulation of Ca2+-induced calcium-release in the heart through the interaction with several Ca2+ channels such as the ryanodine receptor. The modulating activity is impaired in the recently described natural F112L mutant. The F112 residue is located at the end of the D helix next to Asp113, one of the calcium ligands in the EF3 hand endowed with the highest affinity for the metal. The F112L-sorcin X-ray crystal structure at 2.5 A resolution displays marked alterations in the EF3 hand, where the hydrogen bonding network established by Phe112 is disrupted, and in the EF1 region, which is tilted in both monomers that give rise to the dimer, the stable form of the molecule. In turn, the observed tilt is indicative of an increased flexibility of the N-terminal part of the molecule. The structural alterations result in a 6-fold decrease in calcium affinity with respect to the wild-type protein and to an even larger impairment of the interaction with annexin VII and of the ability of sorcin to interact with and inhibit ryanodine receptors. These results provide a plausible structural and functional framework that helps elucidate the phenotypic alterations of mice overexpressing F112L-sorcin.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor., Franceschini S, Ilari A, Verzili D, Zamparelli C, Antaramian A, Rueda A, Valdivia HH, Chiancone E, Colotti G, FASEB J. 2008 Jan;22(1):295-306. Epub 2007 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17699613 17699613]
Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor., Franceschini S, Ilari A, Verzili D, Zamparelli C, Antaramian A, Rueda A, Valdivia HH, Chiancone E, Colotti G, FASEB J. 2008 Jan;22(1):295-306. Epub 2007 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17699613 17699613]
The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein., Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E, J Mol Biol. 2002 Mar 29;317(3):447-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11922676 11922676]
Crystal structure of calcium-free human sorcin: a member of the penta-EF-hand protein family., Xie X, Dwyer MD, Swenson L, Parker MH, Botfield MC, Protein Sci. 2001 Dec;10(12):2419-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11714909 11714909]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Natural f112l sorcin mutant]]
[[Category: Natural f112l sorcin mutant]]
[[Category: Ryanodine receptor interacting protein]]
[[Category: Ryanodine receptor interacting protein]]
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