2jdf: Difference between revisions

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[[Image:2jdf.jpg|left|200px]]
{{Seed}}
[[Image:2jdf.png|left|200px]]


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{{STRUCTURE_2jdf|  PDB=2jdf  |  SCENE=  }}  
{{STRUCTURE_2jdf|  PDB=2jdf  |  SCENE=  }}  


'''HUMAN GAMMA-B CRYSTALLIN'''
===HUMAN GAMMA-B CRYSTALLIN===




==Overview==
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The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.
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{{ABSTRACT_PUBMED_17628592}}


==About this Structure==
==About this Structure==
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[[Category: Polymorphism]]
[[Category: Polymorphism]]
[[Category: Structural protein]]
[[Category: Structural protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:44:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:34:30 2008''

Revision as of 00:34, 29 July 2008

File:2jdf.png

Template:STRUCTURE 2jdf

HUMAN GAMMA-B CRYSTALLIN

Template:ABSTRACT PUBMED 17628592

About this Structure

2JDF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein., Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U, J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592

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