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| [[Image:2jmy.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2jmy| PDB=2jmy | SCENE= }} | | {{STRUCTURE_2jmy| PDB=2jmy | SCENE= }} |
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| '''Solution structure of CM15 in DPC micelles'''
| | ===Solution structure of CM15 in DPC micelles=== |
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| ==Overview==
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| Many antimicrobial peptides form alpha-helices when bound to a membrane. In addition, around 80% of residues in membrane-bound proteins are found in alpha-helical regions. The orientation and location of such helical peptides and proteins in the membrane are key factors determining their function and activity. Here we present a new solution state NMR method for obtaining the orientation of helical peptides in a membrane-mimetic environment (micelle-bound) without any chemical perturbation of the peptide-micelle system. By monitoring proton longitudinal relaxation rates upon addition of the freely water-soluble and inert paramagnetic probe Gd(DTPA-BMA) to an alpha-helical peptide, a wavelike pattern with a periodicity of 3.6 residues per turn is observed. The tilt and azimuth (rotation) angle of the helix determine the shape of this paramagnetic relaxation wave and can be obtained by least-square fitting of measured relaxation enhancements. Results are presented for the 15-residue antimicrobial peptide CM15 which forms an amphipathic helix almost parallel to the surface of the micelle. Thus, a few fast experiments enable the identification of helical regions and determination of the helix orientation within the micelle without the need for covalent modification, isotopic labeling, or sophisticated equipment. This approach opens a path toward the topology determination of alpha-helical membrane-proteins without the need for a complete NOE-based structure determination.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17397158}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17397158 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17397158}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMY OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMY OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Dpc micelle]] | | [[Category: Dpc micelle]] |
| [[Category: Nmr]] | | [[Category: Nmr]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:03:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:23:16 2008'' |
