2jpo: Difference between revisions

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[[Image:2jpo.gif|left|200px]]
{{Seed}}
[[Image:2jpo.png|left|200px]]


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{{STRUCTURE_2jpo|  PDB=2jpo  |  SCENE=  }}  
{{STRUCTURE_2jpo|  PDB=2jpo  |  SCENE=  }}  


'''NMR structure of Antheraea polyphemus pheromone-binding protein 1 at pH 4.5'''
===NMR structure of Antheraea polyphemus pheromone-binding protein 1 at pH 4.5===




==Overview==
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The NMR structure of the Antheraea polyphemus pheromone-binding protein 1 at pH 4.5, ApolPBP1A, was determined at 20 degrees C. The structure consists of six alpha-helices, which are arranged in a globular fold that encapsulates a central helix alpha7 formed by the C-terminal polypeptide segment 131-142. The 3D arrangement of these helices is anchored by the three disulfide bonds 19-54, 50-108 and 97-117, which were identified by NMR. Superposition of the ApolPBP1A structure with the structure of the homologous pheromone-binding protein of Bombyx mori at pH 4.5, BmorPBPA, yielded an rmsd of 1.7 A calculated for the backbone heavy-atoms N, Calpha and C' of residues 10-142. In contrast, the present ApolPBP1A structure is different from a recently proposed molecular model for a low-pH form of ApolPBP1 that does not contain the central helix alpha7. ApolPBP1 exhibits a pH-dependent transition between two different globular conformations in slow exchange on the NMR chemical shift timescale similar to BmorPBP, suggesting that the two proteins use the same mechanism of ligand binding and ejection. The extensive sequence homology observed for pheromone-binding proteins from moth species further implies that the previously proposed mechanism of ligand ejection involving the insertion of a C-terminal helix into the pheromone-binding site is a general feature of pheromone signaling in moths.
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{{ABSTRACT_PUBMED_17884092}}


==About this Structure==
==About this Structure==
2JPO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPO OCA].  
2JPO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPO OCA].  


==Reference==
==Reference==
Structural basis of ligand binding and release in insect pheromone-binding proteins: NMR structure of Antheraea polyphemus PBP1 at pH 4.5., Damberger FF, Ishida Y, Leal WS, Wuthrich K, J Mol Biol. 2007 Nov 2;373(4):811-9. Epub 2007 Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17884092 17884092]
Structural basis of ligand binding and release in insect pheromone-binding proteins: NMR structure of Antheraea polyphemus PBP1 at pH 4.5., Damberger FF, Ishida Y, Leal WS, Wuthrich K, J Mol Biol. 2007 Nov 2;373(4):811-9. Epub 2007 Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17884092 17884092]
Protein NMR structure determination with automated NOE-identification in the NOESY spectra using the new software ATNOS., Herrmann T, Guntert P, Wuthrich K, J Biomol NMR. 2002 Nov;24(3):171-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12522306 12522306]
Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA., Herrmann T, Guntert P, Wuthrich K, J Mol Biol. 2002 May 24;319(1):209-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12051947 12051947]
Torsion angle dynamics for NMR structure calculation with the new program DYANA., Guntert P, Mumenthaler C, Wuthrich K, J Mol Biol. 1997 Oct 17;273(1):283-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9367762 9367762]
The new program OPAL for molecular dynamics simulations and energy refinements of biological macromolecules., Luginbuhl P, Guntert P, Billeter M, Wuthrich K, J Biomol NMR. 1996 Sep;8(2):136-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8914272 8914272]
[[Category: Antheraea polyphemus]]
[[Category: Antheraea polyphemus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Ph-dependent conformation]]
[[Category: Ph-dependent conformation]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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