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| [[Image:2jt7.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }} | | {{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }} |
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| '''NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex'''
| | ===NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex=== |
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| ==Overview==
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| The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure. | | The line below this paragraph, {{ABSTRACT_PUBMED_18052170}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18052170 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18052170}} |
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| ==About this Structure== | | ==About this Structure== |
| 2JT7 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA]. | | 2JT7 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Telomere]] | | [[Category: Telomere]] |
| [[Category: Tggggt]] | | [[Category: Tggggt]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:16:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:57:01 2008'' |
