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| {{STRUCTURE_2nqp| PDB=2nqp | SCENE= }} | | {{STRUCTURE_2nqp| PDB=2nqp | SCENE= }} |
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| '''Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA'''
| | ===Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA=== |
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| ==Overview==
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| Translational accuracy and efficiency depend upon modification of uridines in the tRNA anticodon stem loop (ASL) by a highly conserved pseudouridine synthase TruA. TruA specifically modifies uridines at positions 38, 39, and/or 40 of tRNAs with highly divergent sequences and structures through a poorly characterized mechanism that differs from previously studied RNA-modifying enzymes. The molecular basis for the site and substrate "promiscuity" was studied by determining the crystal structures of E. coli TruA in complex with two different leucyl tRNAs in conjunction with functional assays and computer simulation. The structures capture three stages of the TruA*tRNA reaction, revealing the mechanism by which TruA selects the target site. We propose that TruA utilizes the intrinsic flexibility of the ASL for site promiscuity and also to select against intrinsically stable tRNAs to avoid their overstabilization through pseudouridylation, thereby maintaining the balance between the flexibility and stability required for its biological function.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17466622}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17466622 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17466622}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Pseudouridine synthase]] | | [[Category: Pseudouridine synthase]] |
| [[Category: Trna]] | | [[Category: Trna]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:47:25 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:18:27 2008'' |
