1cgp: Difference between revisions
New page: left|200px<br /> <applet load="1cgp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgp, resolution 3.000Å" /> '''CATABOLITE GENE AC... |
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[[Image:1cgp.gif|left|200px]]<br /> | [[Image:1cgp.gif|left|200px]]<br /><applet load="1cgp" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1cgp, resolution 3.000Å" /> | caption="1cgp, resolution 3.000Å" /> | ||
'''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'''<br /> | '''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'''<br /> | ||
==Overview== | ==Overview== | ||
The 3 angstrom resolution crystal structure of the Escherichia coli | The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation. | ||
==About this Structure== | ==About this Structure== | ||
1CGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1CGP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http:// | 1CGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1CGP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Schultz, S | [[Category: Schultz, S C.]] | ||
[[Category: Shields, G | [[Category: Shields, G C]] | ||
[[Category: Steitz, T | [[Category: Steitz, T A.]] | ||
[[Category: CMP]] | [[Category: CMP]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:53 2008'' | ||
Revision as of 10:05, 21 February 2008
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CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Overview
The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.
About this Structure
1CGP is a Single protein structure of sequence from Escherichia coli with CMP as ligand. The following page contains interesting information on the relation of 1CGP with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:1653449
Page seeded by OCA on Thu Feb 21 12:05:53 2008
