2o1i: Difference between revisions

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[[Image:2o1i.~~gif~~|left|200px]]

[[Image:2o1i.png|left|200px]]

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~~'''~~RH(BPY)2CHRYSI complexed to mismatched DNA~~'''~~

===RH(BPY)2CHRYSI complexed to mismatched DNA===

~~==Overview==~~

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~~We report~~ the ~~1.1-A resolution crystal structure of a bulky rhodium complex bound~~ to ~~two different DNA sites, mismatched and matched in~~ the ~~oligonucleotide 5'-~~(~~dCGGAAATTCCCG)2-3'~~. ~~At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized~~. ~~This unique binding mode contrasts with major groove intercalation, observed at a matched site~~, where ~~doubling of the base pair rise accommodates stacking of~~ the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches.

The line below this paragraph, {{ABSTRACT_PUBMED_17194756}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17194756 is the PubMed ID number.

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==About this Structure==

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[[Category: Dna recognition]]

[[Category: Metallointercalator]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4~~ 10:~~11:32~~ 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:10:55 2008''

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-[[Image:2o1i.gif|left|200px]]
+{{Seed}}
+[[Image:2o1i.png|left|200px]]
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 {{STRUCTURE_2o1i|  PDB=2o1i  |  SCENE=  }}
-'''RH(BPY)2CHRYSI complexed to mismatched DNA'''
+===RH(BPY)2CHRYSI complexed to mismatched DNA===
-==Overview==
+<!--
-We report the 1.1-A resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in the oligonucleotide 5'-(dCGGAAATTCCCG)2-3'. At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized. This unique binding mode contrasts with major groove intercalation, observed at a matched site, where doubling of the base pair rise accommodates stacking of the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches.
+The line below this paragraph, {{ABSTRACT_PUBMED_17194756}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17194756 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17194756}}
 ==About this Structure==
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 [[Category: Dna recognition]]
 [[Category: Metallointercalator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:11:32 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:10:55 2008''