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| {{STRUCTURE_2o49| PDB=2o49 | SCENE= }} | | {{STRUCTURE_2o49| PDB=2o49 | SCENE= }} |
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| '''Crystal Structure of the N-terminal CUT domain of SATB1 Bound to Matrix Attachment Region DNA'''
| | ===Crystal Structure of the N-terminal CUT domain of SATB1 Bound to Matrix Attachment Region DNA=== |
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| ==Overview==
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| Special AT-rich sequence binding protein 1 (SATB1) regulates gene expression essential in immune T-cell maturation and switching of fetal globin species, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin remodeling. Previously we have revealed a five-helix structure of the N-terminal CUT domain, which is essentially the folded region in the MAR-binding domain, of human SATB1 by NMR. Here we determined crystal structure of the complex of the CUT domain and a MAR DNA, in which the third helix of the CUT domain deeply enters the major groove of DNA in the B-form. Bases of 5'-CTAATA-3' sequence are contacted by this helix, through direct and water-mediated hydrogen bonds and apolar and van der Waals contacts. Mutations at conserved base-contacting residues, Gln402 and Gly403, reduced the DNA-binding activity, which confirmed the importance of the observed interactions involving these residues. A significant number of equivalent contacts are observed also for typically four-helix POU-specific domains of POU-homologous proteins, indicating that these domains share a common framework of the DNA-binding mode, recognizing partially similar DNA sequences.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17652321}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17652321 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17652321}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Transcription]] | | [[Category: Transcription]] |
| [[Category: Transcription/dna complex]] | | [[Category: Transcription/dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:18:28 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:46:49 2008'' |
