2o8v: Difference between revisions

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[[Image:2o8v.gif|left|200px]]
{{Seed}}
[[Image:2o8v.png|left|200px]]


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{{STRUCTURE_2o8v|  PDB=2o8v  |  SCENE=  }}  
{{STRUCTURE_2o8v|  PDB=2o8v  |  SCENE=  }}  


'''PAPS reductase in a covalent complex with thioredoxin C35A'''
===PAPS reductase in a covalent complex with thioredoxin C35A===




==Overview==
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The crystal structure of Escherichia coli 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase in complex with E. coli thioredoxin 1 (Trx1) has been determined to 3.0 A resolution. The two proteins are covalently linked via a mixed disulfide that forms during nucleophilic attack of Trx's N-terminal cysteine on the Sgamma atom of the PAPS reductase S-sulfocysteine (E-Cys-Sgamma-SO3-), a central intermediate in the catalytic cycle. For the first time in a crystal structure, residues 235-244 in the PAPS reductase C-terminus are observed, depicting an array of interprotein salt bridges between Trx and the strictly conserved glutathione-like sequence, Glu238Cys239Gly240Leu241His242. The structure also reveals a Trx-binding surface adjacent to the active site cleft and regions of PAPS reductase associated with conformational change. Interaction at this site strategically positions Trx to bind the S-sulfated C-terminus and addresses the mechanism for requisite structural rearrangement of this domain. An apparent sulfite-binding pocket at the protein-protein interface explicitly orients the S-sulfocysteine Sgamma atom for nucleophilic attack in a subsequent step. Taken together, the structure of PAPS reductase in complex with Trx highlights the large structural rearrangement required to accomplish sulfonucleotide reduction and suggests a role for Trx in catalysis beyond the paradigm of disulfide reduction.
The line below this paragraph, {{ABSTRACT_PUBMED_17352498}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_17352498}}


==About this Structure==
==About this Structure==
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[[Category: Stout, C D.]]
[[Category: Stout, C D.]]
[[Category: Disulfide crosslinked complex]]
[[Category: Disulfide crosslinked complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:28:14 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:15:06 2008''

Revision as of 20:15, 28 July 2008

File:2o8v.png

Template:STRUCTURE 2o8v

PAPS reductase in a covalent complex with thioredoxin C35A

Template:ABSTRACT PUBMED 17352498

About this Structure

2O8V is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle., Chartron J, Shiau C, Stout CD, Carroll KS, Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:17352498

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