1lb2: Difference between revisions
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New page: left|200px<br /> <applet load="1lb2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lb2, resolution 3.1Å" /> '''Structure of the E. ... |
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[[Image:1lb2.gif|left|200px]]<br /> | [[Image:1lb2.gif|left|200px]]<br /><applet load="1lb2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1lb2" size=" | |||
caption="1lb2, resolution 3.1Å" /> | caption="1lb2, resolution 3.1Å" /> | ||
'''Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA'''<br /> | '''Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA'''<br /> | ||
==Overview== | ==Overview== | ||
The Escherichia coli catabolite activator protein (CAP) activates | The Escherichia coli catabolite activator protein (CAP) activates transcription at P(lac), P(gal), and other promoters through interactions with the RNA polymerase alpha subunit carboxyl-terminal domain (alphaCTD). We determined the crystal structure of the CAP-alphaCTD-DNA complex at a resolution of 3.1 angstroms. CAP makes direct protein-protein interactions with alphaCTD, and alphaCTD makes direct protein-DNA interactions with the DNA segment adjacent to the DNA site for CAP. There are no large-scale conformational changes in CAP and alphaCTD, and the interface between CAP and alphaCTD is small. These findings are consistent with the proposal that activation involves a simple "recruitment" mechanism. | ||
==About this Structure== | ==About this Structure== | ||
1LB2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LB2 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | 1LB2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LB2 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Benoff, B.]] | [[Category: Benoff, B.]] | ||
[[Category: Berman, H | [[Category: Berman, H M.]] | ||
[[Category: Blatter, E.]] | [[Category: Blatter, E.]] | ||
[[Category: Ebright, R | [[Category: Ebright, R H.]] | ||
[[Category: Ebright, Y | [[Category: Ebright, Y W.]] | ||
[[Category: Lawson, C | [[Category: Lawson, C L.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Parkinson, G.]] | [[Category: Parkinson, G.]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:15 2008'' | ||
