1muh: Difference between revisions
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New page: left|200px<br /> <applet load="1muh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1muh, resolution 2.30Å" /> '''CRYSTAL STRUCTURE O... |
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[[Image:1muh.gif|left|200px]]<br /> | [[Image:1muh.gif|left|200px]]<br /><applet load="1muh" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1muh" size=" | |||
caption="1muh, resolution 2.30Å" /> | caption="1muh, resolution 2.30Å" /> | ||
'''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA'''<br /> | '''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA'''<br /> | ||
==Overview== | ==Overview== | ||
Genomic evolution has been profoundly influenced by DNA transposition, a | Genomic evolution has been profoundly influenced by DNA transposition, a process whereby defined DNA segments move freely about the genome. Transposition is mediated by transposases, and similar events are catalyzed by retroviral integrases such as human immunodeficiency virus-1 (HIV-1) integrase. Understanding how these proteins interact with DNA is central to understanding the molecular basis of transposition. We report the three-dimensional structure of prokaryotic Tn5 transposase complexed with Tn5 transposon end DNA determined to 2.3 angstrom resolution. The molecular assembly is dimeric, where each double-stranded DNA molecule is bound by both protein subunits, orienting the transposon ends into the active sites. This structure provides a molecular framework for understanding many aspects of transposition, including the binding of transposon end DNA by one subunit and cleavage by a second, cleavage of two strands of DNA by a single active site via a hairpin intermediate, and strand transfer into target DNA. | ||
==About this Structure== | ==About this Structure== | ||
1MUH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | 1MUH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1F3I. The following page contains interesting information on the relation of 1MUH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb84_1.html Transposase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUH OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transposase]] | [[Category: Transposase]] | ||
[[Category: Davies, D | [[Category: Davies, D R.]] | ||
[[Category: Goryshin, I | [[Category: Goryshin, I Y.]] | ||
[[Category: Holden, H | [[Category: Holden, H M.]] | ||
[[Category: Rayment, I.]] | [[Category: Rayment, I.]] | ||
[[Category: Reznikoff, W | [[Category: Reznikoff, W S.]] | ||
[[Category: Thoden, J | [[Category: Thoden, J B.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: transposase]] | [[Category: transposase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:06 2008'' | ||
