2oeh: Difference between revisions

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[[Image:2oeh.gif|left|200px]]
{{Seed}}
[[Image:2oeh.png|left|200px]]


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{{STRUCTURE_2oeh|  PDB=2oeh  |  SCENE=  }}  
{{STRUCTURE_2oeh|  PDB=2oeh  |  SCENE=  }}  


'''Determination of the Three-dimensional Structure of the Mrf2-DNA Complex Using Paramagnetic Spin Labeling'''
===Determination of the Three-dimensional Structure of the Mrf2-DNA Complex Using Paramagnetic Spin Labeling===




==Overview==
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Understanding the mechanism of protein-DNA interactions at the molecular level is one of the main focuses in structural and molecular biological investigations. At present, NMR spectroscopy is the only approach that can provide atomic details of protein-DNA recognition in solution. However, determining the structures of protein-DNA complexes using NMR spectroscopy has been dependent on the observation of intermolecular nuclear Overhauser effects (NOE) and their assignments, which are difficult to obtain in many cases. In this study, we have shown that intermolecular distance constraints derived from a single spin-label in combination with docking calculations have defined many specific contacts of the complex between the AT-rich interaction domain (ARID) of Mrf2 and its target DNA. Mrf2 contacts DNA mainly using the two flexible loops, L1 and L2. While the L1 loop contacts the phosphate backbone, L2 and several residues in the adjacent helices interact with AT base pairs in the major groove of DNA. Despite the structural diversity in the ARID family of DNA-binding proteins, Mrf2 maintains contacts with DNA similar to those observed in the homologous Dri-DNA complex.
The line below this paragraph, {{ABSTRACT_PUBMED_17407261}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17407261 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17407261}}


==About this Structure==
==About this Structure==
2OEH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OEH OCA].  
2OEH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OEH OCA].  


==Reference==
==Reference==
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[[Category: Spin labeling]]
[[Category: Spin labeling]]
[[Category: Transcription/dna complex]]
[[Category: Transcription/dna complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:44:26 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:26:27 2008''

Revision as of 12:26, 29 July 2008

File:2oeh.png

Template:STRUCTURE 2oeh

Determination of the Three-dimensional Structure of the Mrf2-DNA Complex Using Paramagnetic Spin Labeling

Template:ABSTRACT PUBMED 17407261

About this Structure

2OEH is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Determination of the three-dimensional structure of the Mrf2-DNA complex using paramagnetic spin labeling., Cai S, Zhu L, Zhang Z, Chen Y, Biochemistry. 2007 May 1;46(17):4943-50. Epub 2007 Apr 4. PMID:17407261

Page seeded by OCA on Tue Jul 29 15:26:27 2008

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