1tbd: Difference between revisions
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New page: left|200px<br /> <applet load="1tbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tbd" /> '''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDIN... |
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[[Image:1tbd.gif|left|200px]]<br /> | [[Image:1tbd.gif|left|200px]]<br /><applet load="1tbd" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDING DOMAIN OF SV40 T-ANTIGEN, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDING DOMAIN OF SV40 T-ANTIGEN, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of the domain from simian virus 40 (SV40) large T-antigen | The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA. | ||
==About this Structure== | ==About this Structure== | ||
1TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. The following page contains interesting information on the relation of 1TBD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb47_1.html Simian Virus 40]]. Full crystallographic information is available from [http:// | 1TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. The following page contains interesting information on the relation of 1TBD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb47_1.html Simian Virus 40]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBD OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bachovchin, W | [[Category: Bachovchin, W W.]] | ||
[[Category: Bullock, P | [[Category: Bullock, P A.]] | ||
[[Category: Luo, X.]] | [[Category: Luo, X.]] | ||
[[Category: Sanford, D | [[Category: Sanford, D G.]] | ||
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
[[Category: replication origin binding domain]] | [[Category: replication origin binding domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:52 2008'' | ||
