1yyf: Difference between revisions

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New page: left|200px<br /> <applet load="1yyf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyf, resolution 4.16Å" /> '''Correction of X-ray...
 
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[[Image:1yyf.gif|left|200px]]<br />
[[Image:1yyf.gif|left|200px]]<br /><applet load="1yyf" size="350" color="white" frame="true" align="right" spinBox="true"  
<applet load="1yyf" size="450" color="white" frame="true" align="right" spinBox="true"  
caption="1yyf, resolution 4.16&Aring;" />
caption="1yyf, resolution 4.16&Aring;" />
'''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects'''<br />
'''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects'''<br />


==Overview==
==Overview==
Because of lattice-translocation defects, two identical but translated, lattices can coexist as a single coherent mosaic block in a crystal. The, observed structure in such cases is a weighted sum of two identical but, translated structures, one from each lattice; the observed structure, factors are a weighted vector sum of the structure factors with identical, unit amplitudes but shifted phases. The correction of X-ray intensities, from a single crystal containing these defects of the hybrid HslV-HslU, complex, which consists of Escherichia coli HslU and Bacillus subtilis, HslV (also known as CodW), is reported. When intensities are not, corrected, a biologically irrelevant complex (with CodW from one lattice, and HslU from another) is implied to exist. Only upon correction does a, biologically functional CodW-HslU complex structure emerge.
Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.


==About this Structure==
==About this Structure==
1YYF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA].  
1YYF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA].  


==Reference==
==Reference==
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Eom, S.H.]]
[[Category: Eom, S H.]]
[[Category: Park, H.H.]]
[[Category: Park, H H.]]
[[Category: Rho, S.H.]]
[[Category: Rho, S H.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
[[Category: ADP]]
[[Category: ADP]]
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[[Category: quaternary structure]]
[[Category: quaternary structure]]


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