2onj: Difference between revisions

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[[Image:2onj.gif|left|200px]]<br />

[[Image:2onj.gif|left|200px]]<br /><applet load="2onj" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2onj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2onj, resolution 3.400Å" />

'''Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP'''<br />

==Overview==

Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC, transporter Mdr1 that causes multidrug resistance in cancer cells. We, report the crystal structure of Sav1866 in complex with, adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution, and compare it with the previously determined structure of Sav1866 with, bound ADP. Besides differences in the ATP-binding sites, no significant, conformational changes were observed. The results confirm that the, ATP-bound state of multidrug ABC transporters is coupled to an, outward-facing conformation of the transmembrane domains.

Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains.

==About this Structure==

2ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NA and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2ONJ OCA].

2ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONJ OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Staphylococcus aureus]]

[[Category: Dawson, R.J.P.]]

[[Category: Dawson, R J.P.]]

[[Category: Locher, K.P.]]

[[Category: Locher, K P.]]

[[Category: ANP]]

[[Category: NA]]

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[[Category: transport protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov~~ 18 09:09:~~16 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:20:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2onj.gif|left|200px]]<br />
+[[Image:2onj.gif|left|200px]]<br /><applet load="2onj" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2onj" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2onj, resolution 3.400&Aring;" />
 '''Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP'''<br />
 ==Overview==
-Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC, transporter Mdr1 that causes multidrug resistance in cancer cells. We, report the crystal structure of Sav1866 in complex with, adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution, and compare it with the previously determined structure of Sav1866 with, bound ADP. Besides differences in the ATP-binding sites, no significant, conformational changes were observed. The results confirm that the, ATP-bound state of multidrug ABC transporters is coupled to an, outward-facing conformation of the transmembrane domains.
+Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains.
 ==About this Structure==
-ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NA and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ONJ OCA].
+ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONJ OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Dawson, R.J.P.]]
+[[Category: Dawson, R J.P.]]
-[[Category: Locher, K.P.]]
+[[Category: Locher, K P.]]
 [[Category: ANP]]
 [[Category: NA]]
@@ Line 21: / Line 20: @@
 [[Category: transport protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:09:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:20:26 2008''