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| {{STRUCTURE_2oqc| PDB=2oqc | SCENE= }} | | {{STRUCTURE_2oqc| PDB=2oqc | SCENE= }} |
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| '''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
| | ===Crystal Structure of Penicillin V acylase from Bacillus subtilis=== |
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| ==Overview==
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| Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.
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| ==About this Structure== | | ==About this Structure== |
| 2OQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. | | 2OQC is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. |
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| ==Reference== | | ==Reference== |
| Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511127 16511127]
| | <ref group="xtra">PMID:16511127</ref><references group="xtra"/> |
| [[Category: Bacillus subtilis]] | | [[Category: Bacillus subtilis]] |
| [[Category: Penicillin amidase]] | | [[Category: Penicillin amidase]] |
| [[Category: Single protein]]
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| [[Category: Brannigan, J A.]] | | [[Category: Brannigan, J A.]] |
| [[Category: Dodson, G G.]] | | [[Category: Dodson, G G.]] |
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| [[Category: Ntn-hydrolase]] | | [[Category: Ntn-hydrolase]] |
| [[Category: Penicillin v acylase]] | | [[Category: Penicillin v acylase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:26:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:59:48 2009'' |
