2oqn: Difference between revisions

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~~'''~~High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths~~'''~~

===High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths===

~~==Overview==~~

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~~Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently~~, ~~a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without~~ the ~~use of penetrative cryoprotectants. Three new developments of~~ the ~~method are presented here~~: (i) ~~Xe-He high-pressure cryocooling for Xe SAD phasing~~, ~~(ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than~~ the ~~native mother liquor~~. ~~These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high~~-~~throughput protein crystallography.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17452791}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17452791 is the PubMed ID number.

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==About this Structure==

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[[Category: High pressure cryocooling]]

[[Category: Sulfur sad phasing]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 11~~:28:10 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:01:50 2008''

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-[[Image:2oqn.jpg|left|200px]]
+{{Seed}}
+[[Image:2oqn.png|left|200px]]
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 {{STRUCTURE_2oqn|  PDB=2oqn  |  SCENE=  }}
-'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
+===High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths===
-==Overview==
+<!--
-Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
+The line below this paragraph, {{ABSTRACT_PUBMED_17452791}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17452791 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17452791}}
 ==About this Structure==
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 [[Category: High pressure cryocooling]]
 [[Category: Sulfur sad phasing]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:28:10 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:01:50 2008''