2otb: Difference between revisions

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[[Image:2otb.jpg|left|200px]]
{{Seed}}
[[Image:2otb.png|left|200px]]


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{{STRUCTURE_2otb|  PDB=2otb  |  SCENE=  }}  
{{STRUCTURE_2otb|  PDB=2otb  |  SCENE=  }}  


'''Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state'''
===Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state===




==Overview==
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Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.
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{{ABSTRACT_PUBMED_17420458}}


==About this Structure==
==About this Structure==
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[[Category: Beta can]]
[[Category: Beta can]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:36:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:33:01 2008''

Revision as of 06:33, 28 July 2008

File:2otb.png

Template:STRUCTURE 2otb

Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state

Template:ABSTRACT PUBMED 17420458

About this Structure

2OTB is a Single protein structure of sequence from Clavularia sp.. Full crystallographic information is available from OCA.

Reference

Structural basis for reversible photobleaching of a green fluorescent protein homologue., Henderson JN, Ai HW, Campbell RE, Remington SJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458

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