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| {{STRUCTURE_2ote| PDB=2ote | SCENE= }} | | {{STRUCTURE_2ote| PDB=2ote | SCENE= }} |
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| '''Crystal structure of a monomeric cyan fluorescent protein in the photobleached state'''
| | ===Crystal structure of a monomeric cyan fluorescent protein in the photobleached state=== |
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| ==Overview==
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| Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17420458}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17420458 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17420458}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta can]] | | [[Category: Beta can]] |
| [[Category: Fluorescent protein]] | | [[Category: Fluorescent protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:37:11 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:44:37 2008'' |
