1c5b: Difference between revisions

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[[Image:1c5b.gif|left|200px]]<br />

[[Image:1c5b.gif|left|200px]]<br /><applet load="1c5b" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1c5b" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1c5b, resolution 2.10Å" />

'''DECARBOXYLASE CATALYTIC ANTIBODY 21D8 UNLIGANDED FORM'''<br />

==Overview==

Antibody 21D8 catalyzes the solvent-sensitive decarboxylation of, 3-carboxybenzisoxazoles. The crystal structure of chimeric Fab 21D8 with, and without hapten at 1.61 A and 2.10 A, respectively, together with, computational analysis, shows how a melange of polar and non-polar sites, are exploited to achieve both substrate binding and acceleration of a, reaction normally facilitated by purely aprotic dipolar media. The, striking similarity of the decarboxylase and a series of unrelated, esterase antibodies also highlights the chemical versatility of, structurally conserved anion binding sites and the relatively subtle, changes involved in fine-tuning the immunoglobulin pocket for recognition, of different ligands and catalysis of different reactions.

Antibody 21D8 catalyzes the solvent-sensitive decarboxylation of 3-carboxybenzisoxazoles. The crystal structure of chimeric Fab 21D8 with and without hapten at 1.61 A and 2.10 A, respectively, together with computational analysis, shows how a melange of polar and non-polar sites are exploited to achieve both substrate binding and acceleration of a reaction normally facilitated by purely aprotic dipolar media. The striking similarity of the decarboxylase and a series of unrelated esterase antibodies also highlights the chemical versatility of structurally conserved anion binding sites and the relatively subtle changes involved in fine-tuning the immunoglobulin pocket for recognition of different ligands and catalysis of different reactions.

==About this Structure==

1C5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_+_homo_sapiens Mus musculus + homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1C5B OCA].

1C5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_+_homo_sapiens Mus musculus + homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5B OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Hotta, K.]]

[[Category: Wilson, I.A.]]

[[Category: Wilson, I A.]]

[[Category: catalytic antibody]]

[[Category: chimeric fab]]

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[[Category: unliganded]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 18 09~~:27:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:31 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1c5b.gif|left|200px]]<br />
+[[Image:1c5b.gif|left|200px]]<br /><applet load="1c5b" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1c5b" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1c5b, resolution 2.10&Aring;" />
 '''DECARBOXYLASE CATALYTIC ANTIBODY 21D8 UNLIGANDED FORM'''<br />
 ==Overview==
-Antibody 21D8 catalyzes the solvent-sensitive decarboxylation of, 3-carboxybenzisoxazoles. The crystal structure of chimeric Fab 21D8 with, and without hapten at 1.61 A and 2.10 A, respectively, together with, computational analysis, shows how a melange of polar and non-polar sites, are exploited to achieve both substrate binding and acceleration of a, reaction normally facilitated by purely aprotic dipolar media. The, striking similarity of the decarboxylase and a series of unrelated, esterase antibodies also highlights the chemical versatility of, structurally conserved anion binding sites and the relatively subtle, changes involved in fine-tuning the immunoglobulin pocket for recognition, of different ligands and catalysis of different reactions.
+Antibody 21D8 catalyzes the solvent-sensitive decarboxylation of 3-carboxybenzisoxazoles. The crystal structure of chimeric Fab 21D8 with and without hapten at 1.61 A and 2.10 A, respectively, together with computational analysis, shows how a melange of polar and non-polar sites are exploited to achieve both substrate binding and acceleration of a reaction normally facilitated by purely aprotic dipolar media. The striking similarity of the decarboxylase and a series of unrelated esterase antibodies also highlights the chemical versatility of structurally conserved anion binding sites and the relatively subtle changes involved in fine-tuning the immunoglobulin pocket for recognition of different ligands and catalysis of different reactions.
 ==About this Structure==
-C5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_+_homo_sapiens Mus musculus + homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C5B OCA].
+C5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_+_homo_sapiens Mus musculus + homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5B OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Hotta, K.]]
-[[Category: Wilson, I.A.]]
+[[Category: Wilson, I A.]]
 [[Category: catalytic antibody]]
 [[Category: chimeric fab]]
@@ Line 22: / Line 21: @@
 [[Category: unliganded]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:27:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:31 2008''