|
|
| Line 1: |
Line 1: |
| [[Image:2p1m.gif|left|200px]] | | {{Seed}} |
| | [[Image:2p1m.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2p1m| PDB=2p1m | SCENE= }} | | {{STRUCTURE_2p1m| PDB=2p1m | SCENE= }} |
|
| |
|
| '''TIR1-ASK1 complex structure'''
| | ===TIR1-ASK1 complex structure=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear. Here we present the crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. These structures show that the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor and recognizes auxin and the Aux/IAA polypeptide substrate through a single surface pocket. Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies the rest of the TIR1 pocket and completely encloses the hormone-binding site. By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17410169}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17410169 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_17410169}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: F-box]] | | [[Category: F-box]] |
| [[Category: Leucine rich repeat]] | | [[Category: Leucine rich repeat]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:09:02 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:37:08 2008'' |
