|
|
| Line 1: |
Line 1: |
| [[Image:2p32.gif|left|200px]] | | {{Seed}} |
| | [[Image:2p32.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2p32| PDB=2p32 | SCENE= }} | | {{STRUCTURE_2p32| PDB=2p32 | SCENE= }} |
|
| |
|
| '''Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70'''
| | ===Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Hsp70 chaperones are composed of two domains; the 40 kDa N-terminal nucleotide-binding domain (NDB) and the 30 kDa C-terminal substrate-binding domain (SBD). Structures of the SBD from Escherichia coli homologues DnaK and HscA show it can be further divided into an 18 kDa beta-sandwich subdomain, which forms the hydrophobic binding pocket, and a 10 kDa C-terminal three-helix bundle that forms a lid over the binding pocket. Across prokaryotes and eukaryotes, the NBD and beta-sandwich subdomain are well conserved in both sequence and structure. The C-terminal subdomain is, however, more evolutionary variable and the only eukaryotic structure from rat Hsc70 revealed a diverged helix-loop-helix fold. We have solved the crystal structure of the C-terminal 10 kDa subdomain from Caenorhabditis elegans Hsp70 which forms a helical-bundle similar to the prokaryotic homologues. This provides the first confirmation of the structural conservation of this subdomain in eukaryotes. Comparison with the rat structure reveals a domain-swap dimerisation mechanism; however, the C. elegans subdomain exists exclusively as a monomer in solution in agreement with the hypothesis that regions out with the C-terminal subdomain are necessary for Hsp70 self-association.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17407764}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17407764 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_17407764}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 25: |
Line 29: |
| [[Category: Worrall, L J.]] | | [[Category: Worrall, L J.]] |
| [[Category: Three-helix bundle]] | | [[Category: Three-helix bundle]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:14:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:51:34 2008'' |
