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| [[Image:2p6w.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2p6w| PDB=2p6w | SCENE= }} | | {{STRUCTURE_2p6w| PDB=2p6w | SCENE= }} |
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| '''Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''
| | ===Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1=== |
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| ==Overview==
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| Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17850743}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17850743 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17850743}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Glycosyltransferase]] | | [[Category: Glycosyltransferase]] |
| [[Category: Pbcv]] | | [[Category: Pbcv]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:30:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:36:20 2008'' |
