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| [[Image:2p7v.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2p7v| PDB=2p7v | SCENE= }} | | {{STRUCTURE_2p7v| PDB=2p7v | SCENE= }} |
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| '''Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4'''
| | ===Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4=== |
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| ==Overview==
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| The Escherichia coli Rsd protein binds tightly and specifically to the RNA polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma factor-dependent transcription by biasing the competition between sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70). The structure reveals that Rsd binding interferes with the two primary functions of sigma(70)(4), core RNAP binding and promoter -35 element binding. Interestingly, the most highly conserved Rsd residues form a network of interactions through the middle of the Rsd structure that connect the sigma(70)(4)-binding surface with three cavities exposed on distant surfaces of Rsd, suggesting functional coupling between sigma(70)(4) binding and other binding surfaces of Rsd, either for other proteins or for as yet unknown small molecule effectors. These results provide a structural basis for understanding the role of Rsd, as well as its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa virulence, in transcription regulation. | | The line below this paragraph, {{ABSTRACT_PUBMED_17681541}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17681541 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17681541}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sigma factor]] | | [[Category: Sigma factor]] |
| [[Category: Transcription]] | | [[Category: Transcription]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:34:18 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:54:47 2008'' |
