|
|
| Line 1: |
Line 1: |
| [[Image:2phm.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2phm.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2phm| PDB=2phm | SCENE= }} | | {{STRUCTURE_2phm| PDB=2phm | SCENE= }} |
|
| |
|
| '''STRUCTURE OF PHENYLALANINE HYDROXYLASE DEPHOSPHORYLATED'''
| | ===STRUCTURE OF PHENYLALANINE HYDROXYLASE DEPHOSPHORYLATED=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Phenylalanine hydroxylase converts phenylalanine to tyrosine, a rate-limiting step in phenylalanine catabolism and protein and neurotransmitter biosynthesis. It is tightly regulated by the substrates phenylalanine and tetrahydrobiopterin and by phosphorylation. We present the crystal structures of dephosphorylated and phosphorylated forms of a dimeric enzyme with catalytic and regulatory properties of the wild-type protein. The structures reveal a catalytic domain flexibly linked to a regulatory domain. The latter consists of an N-terminal autoregulatory sequence (containing Ser 16, which is the site of phosphorylation) that extends over the active site pocket, and an alpha-beta sandwich core that is, unexpectedly, structurally related to both pterin dehydratase and the regulatory domains of metabolic enzymes. Phosphorylation has no major structural effects in the absence of phenylalanine, suggesting that phenylalanine and phosphorylation act in concert to activate the enzyme through a combination of intrasteric and possibly allosteric mechanisms.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10331871}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10331871 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_10331871}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 34: |
Line 38: |
| [[Category: Phenylalanine hydroxylase]] | | [[Category: Phenylalanine hydroxylase]] |
| [[Category: Phosphorylation]] | | [[Category: Phosphorylation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:07:43 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:55:08 2008'' |
