1mfa: Difference between revisions

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[[Image:1mfa.gif|left|200px]]<br />

[[Image:1mfa.gif|left|200px]]<br /><applet load="1mfa" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1mfa" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1mfa, resolution 1.7Å" />

'''STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION'''<br />

==Overview==

We describe here the 1.7-A resolution structure of a single-chain antibody, variable domain (scFv) molecule, based on the carbohydrate-binding, antibody Se155-4, complexed with the trisaccharide ligand, alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is, abequose. The scFv expressed in Escherichia coli has the variable region, light chain to heavy chain polarity with the domains connected by a, 19-residue linker. Although the linker is partially disordered in the, crystal, the packing of the molecules suggests a monomeric state of the, scFv. The carbohydrate adopts a different conformation about the Man-Gal, linkage than was observed previously in the Fab-trisaccharide complex., Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms, are bridged by a water molecule in the present complex.

We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is abequose. The scFv expressed in Escherichia coli has the variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker. Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man-Gal linkage than was observed previously in the Fab-trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.

==About this Structure==

1MFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].

1MFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].

==Reference==

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[[Category: immunoglobulin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 18 09~~:36:~~24 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:43 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1mfa.gif|left|200px]]<br />
+[[Image:1mfa.gif|left|200px]]<br /><applet load="1mfa" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1mfa" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1mfa, resolution 1.7&Aring;" />
 '''STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION'''<br />
 ==Overview==
-We describe here the 1.7-A resolution structure of a single-chain antibody, variable domain (scFv) molecule, based on the carbohydrate-binding, antibody Se155-4, complexed with the trisaccharide ligand, alpha-D-Gal(1--&gt;2)[alpha-D-Abe(1--&gt;3)]alpha-D-Manp1--&gt;OMe, where Abe is, abequose. The scFv expressed in Escherichia coli has the variable region, light chain to heavy chain polarity with the domains connected by a, 19-residue linker. Although the linker is partially disordered in the, crystal, the packing of the molecules suggests a monomeric state of the, scFv. The carbohydrate adopts a different conformation about the Man-Gal, linkage than was observed previously in the Fab-trisaccharide complex., Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms, are bridged by a water molecule in the present complex.
+We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1--&gt;2)[alpha-D-Abe(1--&gt;3)]alpha-D-Manp1--&gt;OMe, where Abe is abequose. The scFv expressed in Escherichia coli has the variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker. Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man-Gal linkage than was observed previously in the Fab-trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.
 ==About this Structure==
-MFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].
+MFA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: immunoglobulin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:36:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:43 2008''