|
|
| Line 1: |
Line 1: |
| [[Image:2pnh.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2pnh.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2pnh| PDB=2pnh | SCENE= }} | | {{STRUCTURE_2pnh| PDB=2pnh | SCENE= }} |
|
| |
|
| '''Escherichia coli PriB E39A variant'''
| | ===Escherichia coli PriB E39A variant=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Collapsed DNA replication forks must be reactivated through origin-independent reloading of the replication machinery (replisome) to ensure complete duplication of cellular genomes. In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome reloading. However, the molecular mechanisms that regulate origin-independent replisome loading are not fully understood. Here, we demonstrate that assembly of primosome protein complexes represents a key regulatory mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are strongly stimulated by single-stranded DNA. Furthermore, the binding site on PriB for single-stranded DNA partially overlaps the binding sites for PriA and DnaT, suggesting a dynamic primosome assembly process in which single-stranded DNA is handed off from one primosome protein to another as a repaired replication fork is reactivated. This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17588514}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17588514 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_17588514}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 27: |
Line 31: |
| [[Category: Dna binding protein]] | | [[Category: Dna binding protein]] |
| [[Category: Ob fold]] | | [[Category: Ob fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:28:49 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:33:11 2008'' |
