2po4: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
[[Image:2po4.jpg|left|200px]]
{{Seed}}
[[Image:2po4.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2po4|  PDB=2po4  |  SCENE=  }}  
{{STRUCTURE_2po4|  PDB=2po4  |  SCENE=  }}  


'''X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase'''
===X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase===




==Overview==
<!--
Coliphage N4 virion RNA polymerase (vRNAP), which is injected into the host upon infection, transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure. Here, we describe the 2.0-A resolution x-ray crystal structure of N4 mini-vRNAP, a member of the T7-like, single-unit RNAP family and the minimal component having all RNAP functions of the full-length vRNAP. The structure resembles a "fisted right hand" with Fingers, Palm and Thumb subdomains connected to an N-terminal domain. We established that the specificity loop extending from the Fingers along with W129 of the N-terminal domain play critical roles in hairpin-promoter recognition. A comparison with the structure of the T7 RNAP initiation complex reveals that the pathway of the DNA to the active site is blocked in the apo-form vRNAP, indicating that vRNAP must undergo a large-scale conformational change upon promoter DNA binding and explaining the highly restricted promoter specificity of vRNAP that is essential for phage early transcription.
The line below this paragraph, {{ABSTRACT_PUBMED_18362338}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 18362338 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_18362338}}


==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Right hand shape]]
[[Category: Right hand shape]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:31:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:56:26 2008''

Revision as of 01:56, 29 July 2008

File:2po4.png

Template:STRUCTURE 2po4

X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase

Template:ABSTRACT PUBMED 18362338

About this Structure

2PO4 is a Single protein structure of sequence from Enterobacteria phage n4. Full crystallographic information is available from OCA.

Reference

X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase., Murakami KS, Davydova EK, Rothman-Denes LB, Proc Natl Acad Sci U S A. 2008 Mar 24;. PMID:18362338

Page seeded by OCA on Tue Jul 29 04:56:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2po4&oldid=727906"