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| {{STRUCTURE_2pqg| PDB=2pqg | SCENE= }} | | {{STRUCTURE_2pqg| PDB=2pqg | SCENE= }} |
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| '''Crystal structure of inactive ribosome inactivating protein from maize (b-32)'''
| | ===Crystal structure of inactive ribosome inactivating protein from maize (b-32)=== |
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| ==Overview==
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| Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 A, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long alpha-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17855394}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17855394 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17855394}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Pro-rip]] | | [[Category: Pro-rip]] |
| [[Category: Ribosome inactivating protein]] | | [[Category: Ribosome inactivating protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:37:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:20:47 2008'' |
